In this paper, two authors of the recently published Metropolitan Science: London Sites and Cultures of Knowledge and Practice, c. 1600-1800 (Bloomsbury, 2024), discuss the book's approach and share insights from selected case studies. The book offers a new perspective on the development of knowledge cultures in early modern London, that focuses on the institutional sites of artisanal, mercantile and governmental bodies. They show how the distinct practices and kinds of knowledge developed within such institutions were shaped by the spaces in which they operated, including the ways in which these sites separated or brought together particular people, skills and materials. They will also consider ways in which these institutional cultures were shaped by the  wider city and, in turn, left their impact on the city itself.